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  • Ontogeny of prolyl endopeptidase and pyroglutamyl peptidase I in rat tissues.

Ontogeny of prolyl endopeptidase and pyroglutamyl peptidase I in rat tissues.

Regulatory peptides (2006-11-25)
Naiara Agirregoitia, Luis Casis, Javier Gil, Fátima Ruiz, Jon Irazusta
ABSTRACT

Prolyl endopeptidase and pyroglutamyl peptidase I are enzymes which participate in the degradation of thyrotropin-releasing hormone (TRH), a hormone which is thought to play an important role in the development of organs and tissues. Here, we have characterized the ontogeny of TRH degrading enzyme activity in the brain cortex, lung, heart, kidney and liver. Overall, prolyl endopeptidase activity was found to be 2 to 5 fold higher in newborn vs. adult rat tissues, with the exception of the soluble form in the liver and the particulate form in the lung. In contrast, the developmental profile of pyroglutamyl peptidase I activity was found to be more variable and tissue dependent. These results corroborate the idea that both enzymes play important, tissue-specific roles during the development and maturation of rat organs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pyroglutamate Aminopeptidase from Pyrococcus furiosus, recombinant from E. coli, 7-13 mU (per vial)
Sigma-Aldrich
Pyroglutamate Aminopeptidase from Pyrococcus furiosus, recombinant, expressed in E. coli, ~90% (SDS-PAGE), ≥5.0 units/mg protein