- Peptide-surface adsorption free energy comparing solution conditions ranging from low to medium salt concentrations.
Peptide-surface adsorption free energy comparing solution conditions ranging from low to medium salt concentrations.
Chemphyschem : a European journal of chemical physics and physical chemistry (2012-10-09)
Yang Wei, Aby A Thyparambil, Robert A Latour
PMID23042700
ABSTRACT
Multi-technique methods involving surface plasmon resonance spectroscopy and atomic force microscopy provide experimental data for the characterization of peptide adsorption on self-assembled monolayers. A comparative study is carried out in phosphate-buffered saline (PBS) and potassium phosphate-buffered (PPB) water to determine the influence of the salt concentration on the adsorption behavior (see figure; ΔG(0)(ads) : free energy of peptide adsorption, F(des) : force required for peptide desorption).
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Potassium phosphate dibasic, anhydrous, for luminescence, for molecular biology, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Potassium phosphate monobasic, BioUltra, for molecular biology, anhydrous, ≥99.5% (T)
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Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
Potassium phosphate monobasic, buffer substance, anhydrous, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.5-100.5%
Sigma-Aldrich
Potassium phosphate monobasic, meets analytical specification of Ph. Eur., NF, E340, anhydrous, 98-100.5% (calc. to the dried substance)