LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.

LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.

Autophagy (2021-11-23)

Tsutomu Tanaka, Blake M Warner, Drew G Michael, Hiroyuki Nakamura, Toshio Odani, Hongen Yin, Tatsuya Atsumi, Masayuki Noguchi, John A Chiorini

PMID34802379

ABSTRACT

A253-control: A253 control for LAMP3 stable overexpression; A253- LAMP3: A253 LAPM3 stable overexpression; CASP1: caspase 1; CASP3: caspase 3; CHX: cycloheximide; CTSB: cathepsin B; CTSD: cathepsin D; CQ: chloroquine; DCs: dendritic cells; ER: endoplasmic reticulum; LGALS3: galectin 3; HCV: hepatitis C virus; HSG-control: HSG control for LAMP3 stable overexpression; HSG-LAMP3: HSG LAMP3 stable overexpression; HSP: heat shock protein; HTLV-1: human T-lymphocyte leukemia virus-1; IXA: ixazomib; LAMP: lysosomal associated membrane protein; MHC: major histocompatibility complex; mAb: monoclonal antibody; OE: overexpression; pepA: pepstatin A; pAb: polyclonal antibody; pSS: primary Sjögren syndrome; qRT-PCR: quantitative real- time reverse transcriptase polymerase chain reaction; SLE: systemic lupus erythematosus; SS: Sjögren syndrome; UPR: unfolded protein response; V-ATPase: vacuolar-type proton- translocating ATPase; Y-VAD: Ac-YVAD-cmk; Z-DEVD; Z-DEVD-fmk; Z-VAD: Z-VAD- fmk.

MATERIALS

Product Number

Brand

Product Description

C6628

Sigma-Aldrich

Chloroquine diphosphate salt, powder or crystals, 98.5-101.0% (EP)

C7698

Sigma-Aldrich

Cycloheximide, from microbial, ≥94% (TLC)

A7638

Sigma-Aldrich

Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

SML0429

Sigma-Aldrich

Ac-YVAD-cmk, ≥95% (HPLC)

264155

Sigma-Aldrich

Caspase-3 Inhibitor II, The Caspase-3 Inhibitor II, also referenced under CAS 210344-95-9, controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

T6199

Sigma-Aldrich

Anti-α-Tubulin antibody, Mouse monoclonal, clone DM1A, purified from hybridoma cell culture