Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath.

Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath.

Science advances (2021-09-16)

Xingjie Ren, Mengchi Wang, Bingkun Li, Kirsty Jamieson, Lina Zheng, Ian R Jones, Bin Li, Maya Asami Takagi, Jerry Lee, Lenka Maliskova, Tsz Wai Tam, Miao Yu, Rong Hu, Lindsay Lee, Armen Abnousi, Gang Li, Yun Li, Ming Hu, Bing Ren, Wei Wang, Yin Shen

PMID34524848

ABSTRACT

[Figure: see text].

MATERIALS

Product Number

Brand

Product Description

D9891

Sigma-Aldrich

Doxycycline hyclate

07-729

Sigma-Aldrich

Anti-CTCF Antibody, serum, Upstate^®

04-745

Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal, culture supernatant, clone MC315, Upstate^®

A4660

Sigma-Aldrich

6-Thioguanine, Hybri-Max^™, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma