Silica modified with polymeric amphiphilic nanoparticles as first dimension for multidimensional separation techniques.

This paper addresses the evaluation of a new amphiphilic nanoparticle supported on silica and its application as sorbent in on-line solid phase extraction. The investigated sorbent material is a copolymer composed by [2- (Acryloyloxy) ethyl] trimethylammonium chloride (block A) and butyl acrylate (block B) prepared by reversible addition-fragmentation chain transfer. After polymerization the nanoparticles were adsorbed into silica surface by electrostatic attraction providing to the sorbent both, apolar and polar characteristics. After the fundamental studies to understand the main features of the synthesized nanoparticles supported on silica, this sorbent material was packed into an extraction column. This column was connected on line with liquid chromatography-tandem mass spectrometry and utilized for automated online determination of several analytes as Ochratoxin A, azoxystrobin, cyproconazole and difenoconazole, in wine and water samples.