- Genetic regulators of large-scale transcriptional signatures in cancer.
Genetic regulators of large-scale transcriptional signatures in cancer.
Nature genetics (2006-03-07)
Adam S Adler, Meihong Lin, Hugo Horlings, Dimitry S A Nuyten, Marc J van de Vijver, Howard Y Chang
PMID16518402
ABSTRACT
Gene expression signatures encompassing dozens to hundreds of genes have been associated with many important parameters of cancer, but mechanisms of their control are largely unknown. Here we present a method based on genetic linkage that can prospectively identify functional regulators driving large-scale transcriptional signatures in cancer. Using this method we show that the wound response signature, a poor-prognosis expression pattern of 512 genes in breast cancer, is induced by coordinate amplifications of MYC and CSN5 (also known as JAB1 or COPS5). This information enabled experimental recapitulation, functional assessment and mechanistic elucidation of the wound signature in breast epithelial cells.
MATERIALS
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Product Description
Sigma-Aldrich
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric, The Cell Invasion Assay Kit kuses a 24 well plate with 8 um pores, which is ideal for evaluation of invasive tumor cells.
Sigma-Aldrich
QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric, The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.