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G005744

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] water; [B] acetonitrile; (5:95, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 10 μL

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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