ZRB2141
Anti-OAS1 Antibody, clone 1N2 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
2′-5′-oligoadenylate synthase 1;EC:2.7.7.84;(2-5′)oligo(A) synthase 1;2-5A synthase 1;E18/E16;p46/p42 OAS
Sign Into View Organizational & Contract Pricing
All Photos(14)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1N2, recombinant monoclonal
description
1N2 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 46.03 kDa
observed mol wt ~52 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... OAS1(4938)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1N2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects 2′-5′-oligoadenylate synthase 1 (OAS1). It targets an epitope within 17 amino acids from the N-terminal half.
Immunogen
KLH conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human 2′-5′-oligoadenylate synthase 1 (OAS1).
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from HeLa cells treated with IFN- .
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected OAS1 in lysate from HeLa cells treated with IFN- .
Tested Applications
Enzyme Immunoassay (ELISA) Analysis: Various dilutions from a representative lot detected OAS1 in ELISA applicaiton.
Affinity Binding Assay: A representative lot of this antibody bound OAS1 peptide with a KD of 1.4 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected OAS1 in Human small intestine tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OAS1 in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in lysate from HeLa cells treated with IFN- .
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected OAS1 in lysate from HeLa cells treated with IFN- .
Tested Applications
Enzyme Immunoassay (ELISA) Analysis: Various dilutions from a representative lot detected OAS1 in ELISA applicaiton.
Affinity Binding Assay: A representative lot of this antibody bound OAS1 peptide with a KD of 1.4 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected OAS1 in Human small intestine tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OAS1 in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
2′-5′-oligoadenylate synthase 1 (UniProt: P00973; also known as EC:2.7.7.84, (2-5′)oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS) is encoded by the OAS1 (also known as OIAS) gene (Gene ID: 4938) in human. OAS is an interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. It is frequently detected in nasal epithelium of healthy individuals and displays antiviral activity. It is involved in the synthesis of higher oligomers of 2′-5′-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L then leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis and termination of viral replication. By activating RNase L, it potently inhibits severe acute SARS-CoV-2. Its C-terminal prenylation (isoform p46) is shown to be essential to initiate a SARS-CoV-2 block. The prenylated form is targeted to perinuclear structures rich in viral dsRNA, whereas the non-prenylated form (isoform p42) is diffusely localized and is unable to initiate a detectable block to SARS-CoV-2 replication. OAS1 also has antiviral effects that are independent of RNase L. OAS1 also plays a role in other cellular processes, including apoptosis, cell growth, differentiation, and gene regulation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wickenhagen, A. et al. (2021). Science. 374(6567); eabj3624).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service