574591

Superoxide Dismutase, Human, Recombinant, E. coli

• Synonym(s): 5-LO, Cu/Zn SOD, Human, Recombinant, E. coli
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥3000 U/mg
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 100
• assay: ≥90% (SDS-PAGE)
• form: solid
• specific activity: ≥3000 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: soluble (Reconstitute in sterile MQ water to a final concentration of >=100 µg/ml. Further dilute in aqueous buffers just prior to use.)
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Recombinant, human superoxide dismutase expressed in E. coli as a homodimer consisting of 2 identical, 154-amino acid chains. Purified by proprietary chromatographic methods. Superoxide dismutase is an essential component of biological defense against the toxic effects of oxygen. It catalyzes the dismutation of superoxide radicals to molecular oxygen and H₂O₂ and plays a role in cellular aging due to its reduced activity in aging cells. Mutant forms of Cu/Zn SOD are associated with neurodegenerative disease.

Physical form

Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Preparation Note

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 2 weeks at 4°C or for up to 4 months at -20°C.

Reconstitute in sterile MQ water to a final concentration of ≥100 µg/ml. Further dilute in aqueous buffers just prior to use.

Other Notes

Baumber, J. and Ball, B.A. 2005. Am. J. Vet. Res.66, 1415.
Dimitrova, A.A., et al. 2005. Folia Med. (Plovdiv)47, 42.
Jebara, S., et al. 2005. J. Plant Physiol.162, 929.
Mila-Kierzenkowska, C., et al. 2005. Acta Biol. Hung.56, 399.
Miszalski, Z., et al. 2005. J. Plant Physiol.162, 895.
Paramonov, B.A., et al. 2005. Bull. Exp. Biol. Med.139, 56.

≥3000 units/mg.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable