Skip to Content
MilliporeSigma


We can synthesize custom siRNA for duplexing using your sequences or sequences we have bioinformatically designed for you. We can also add a variety of chemical modifications tailored to your needs. All siRNAs are analyzed by mass spec prior to release.

Product Benefits

  • Complimentary designs with the Rosetta algorithm*
  • Simplified transfection optimization with 11 Positive Control siRNA
  • Distinguish sequence-specific silencing from non–specific effects with 8 negative control siRNA
  • Custom services allow for flexibility in experimental set up

*The proprietary Rosetta siRNA Design Algorithm utilizes Position-Specific Scoring Matrices and knowledge of the seed region to predict the most specific and effective sequences for your target genes. The algorithm’s rules were developed utilizing empirical data collected from gene silencing experiments carried out over three years.

Custom siRNA Design Request

Custom siRNA Duplex Specifications

**Depending on manufacturing site, PAGE may be used to assess siRNA duplexes

siRNA Duplex Pools***

A popular pooled format is 4 duplexes at 5 nmol each combined into one tube (20 nmol pooled) plus the exact same 4 duplexes also at 5 nmol each in separate tubes (another 20 nmol individual). However, our sophisticated liquid handlers allow for a wide range of other possibilities.

For anything marked as ‘Inquire’ or if you have needs that are different from the other general specifications presented, please send a request to sirnarequest@milliporesigma.com.

MISSION is a trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. Label License.

Order Now
Related Product Categories
PCR Reagents & Kits
Polymerase Chain Reaction (PCR) is a mainstay in molecular biology labs. PCR is used for amplifying DNA by several orders of magnitude and our reagents and resources are suitable for numerous research workflows including, gene expression analysis, genotyping, sequencing, and mutagenesis application needs.
Custom & Predesigned DNA Oligos and qPCR Probes
Discover our custom DNA oligos portfolio including primers, qPCR probes, high-throughput DNA in plates, and next-generation sequencing (NGS) adapters available in multiple formats that comply with ISO 9001 and ISO 13485 quality systems for research and commercial applications.
Oligos, qPCR Probes & Peptides
Choose custom products from our comprehensive portfolio of DNA and RNA oligos, qPCR probes and peptides as we offer numerous options for manufacturing platforms, purity levels, modifications to meet a variety of research applications.
Related Applications
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
qPCR
Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
Gene Expression & Silencing
CRISPR, miRNA, siRNA, and shRNA technologies for gene expression and gene silencing applications.
IVD Manufacturing
A brief overview on the key components involved in successful In vitro diagnostic (IVD) assay development. Learn more on IVD scale up, manufacturing, and commercialization.
Related Services
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?