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  • Septin7 is indispensable for proper skeletal muscle architecture and function.

Septin7 is indispensable for proper skeletal muscle architecture and function.

eLife (2022-08-06)
Mónika Gönczi, Zsolt Ráduly, László Szabó, János Fodor, Andrea Telek, Nóra Dobrosi, Norbert Balogh, Péter Szentesi, Gréta Kis, Miklós Antal, György Trencsenyi, Beatrix Dienes, László Csernoch
ABSTRACT

Today septins are considered as the fourth component of the cytoskeleton, with the Septin7 isoform playing a critical role in the formation of higher-order structures. While its importance has already been confirmed in several intracellular processes of different organs, very little is known about its role in skeletal muscle. Here, using Septin7 conditional knockdown (KD) mouse model, the C2C12 cell line, and enzymatically isolated adult muscle fibers, the organization and localization of septin filaments are revealed, and an ontogenesis-dependent expression of Septin7 is demonstrated. KD mice displayed a characteristic hunchback phenotype with skeletal deformities, reduction in in vivo and in vitro force generation, and disorganized mitochondrial networks. Furthermore, knockout of Septin7 in C2C12 cells resulted in complete loss of cell division while KD cells provided evidence that Septin7 is essential for proper myotube differentiation. These and the transient increase in Septin7 expression following muscle injury suggest that it may be involved in muscle regeneration and development.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse, clone EA-53, ascites fluid
Sigma-Aldrich
Anti-Desmin antibody produced in rabbit, whole antiserum
Sigma-Aldrich
Monoclonal Anti-PAX7, (C-terminal) antibody produced in mouse, clone 1E12, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Collagenase type I, The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.