The molecular weight of the protein as provided is 24.8 kDa.
SRE0015
Salt Active Nuclease (SAN)
recombinant, expressed in Pichia pastoris
Synonym(s):
Endonucelase, Salt active endonuclease
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
fermentation/recombinant
Quality Level
recombinant
expressed in Pichia pastoris
form
buffered aqueous glycerol solution
specific activity
≥250000 U/mg
technique(s)
DNA extraction: suitable
suitability
suitable for nucleic acid purification
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
Related Categories
Application
Salt active nuclease has been used to remove DNA during protein expression and purification.[1]
Biochem/physiol Actions
Salt active nuclease (SAN) is a general, unspecific endonuclease that cleaves double-stranded and single-stranded DNA, and RNA. SAN is active at above neutral pH. Unlike other nucleases, however, it has optimum activity at high concentrations of salt at high pH. These qualities make SAN ideal for removal of DNA from cell extracts and protein samples. The end-products upon complete degradation of DNA consist of a majority of 5 nucleotide
oligos. The enzyme degrades DNA vs. RNA in a ratio of 10:1.
SAN is highly active over the temperature range 10-40 °C. The optimal NaCl-concentration for activity is 0.5 M. However, SAN is active in low salt buffers. 1 M NaCl is not recommended for use of SAN. Mg2+ (>1 mM) is required for activity. The pH working range is above neutral pH, with an optimal range between pH 8.5-9.5
Physical form: Solution in 25 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 0.5 M NaCl, 0.01% Triton, and 50% (v/v) glycerol
Unit definition: One unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37 ºC, using 50 μg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 ºC), 5 mM MgCl2, and 500 mM NaCl.
oligos. The enzyme degrades DNA vs. RNA in a ratio of 10:1.
SAN is highly active over the temperature range 10-40 °C. The optimal NaCl-concentration for activity is 0.5 M. However, SAN is active in low salt buffers. 1 M NaCl is not recommended for use of SAN. Mg2+ (>1 mM) is required for activity. The pH working range is above neutral pH, with an optimal range between pH 8.5-9.5
Physical form: Solution in 25 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 0.5 M NaCl, 0.01% Triton, and 50% (v/v) glycerol
Unit definition: One unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37 ºC, using 50 μg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 ºC), 5 mM MgCl2, and 500 mM NaCl.
Unit Definition
One unit (U) is defined as an increase in absorbance at 260 nm of 1 absorbance unit (AU) in 30 minutes at 37 °C, using 50 μg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 °C), 5 mM MgCl2, and 500 mM NaCl.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Transcriptome engineering with RNA-targeting type VI-D CRISPR effectors
Konermann S, et al.
Cell, 665-676 (2018)
-
What is the molecular weight?
1 answer-
Helpful?
-
-
Hi! Is any of your Salt Active Nuclease (SAN) GMP certified?
1 answer-
Unfortunately, we currently do not offer that grade for this material.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
