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A2901

Avidin–FITC from egg white

lyophilized powder

Synonym(s):

Avidin–Fluorescein isothiocyanate from egg white

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00130585
Form:
lyophilized powder
Pricing and availability is not currently available.
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conjugate

FITC conjugate

Quality Segment

200

form

lyophilized powder

extent of labeling

2-4 mol fluorescein isothiocyanate per mol avidin

technique(s)

immunohistochemistry: suitable

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-FITC from egg white has been used for immunohistochemistry applications[2].
To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.[3]

Packaging

Package size based on protein content

Physical form

Contains sodium citrate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A9275189725A7294
Avidin–FITC from egg white lyophilized powder

Sigma-Aldrich

A2901

Avidin–FITC from egg white

Avidin from egg white BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

Sigma-Aldrich

A9275

Avidin from egg white

Avidin, Egg White

Sigma-Aldrich

189725

Avidin, Egg White

Avidin–Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

A7294

Avidin–Alkaline Phosphatase

technique(s)

immunohistochemistry: suitable

technique(s)

-

technique(s)

-

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

form

lyophilized powder

form

lyophilized powder

form

lyophilized solid

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

conjugate

FITC conjugate

conjugate

-

conjugate

-

conjugate

alkaline phosphatase conjugate

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

extent of labeling

2-4 mol fluorescein isothiocyanate per mol avidin

extent of labeling

-

extent of labeling

-

extent of labeling

-

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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