Centrifuge the vial prior to opening, then reconstitute it in water to a concentration of 0.1-1.0 mg/ml. Do not vortex the mixture. For extended storage, it is recommended to
further, dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
GF346
Transforming Growth Factor-β1 human
Animal free, >98%, recombinant, expressed in CHO cells, suitable for cell culture
Synonym(s):
TGFb1, TGFbI, TGFB, Transforming Growth Factor-β1
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
Pricing and availability is not currently available.
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General description
Product Source: CHO cells
The three mammalian isoforms of TGF-β (TGF-β1, TGF-β2, TGF-β3) signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF- isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors.
Recombinant human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
Recombinant human TGF-β1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Quality
Determined by TGF-β1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The expected ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.
Physical form
Product is presented without additives and is filtered through a 0.2 micron filter before lyophilization
Storage and Stability
Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Instructions for resuspending the recombinant TGFb for tissue culture applications
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