1.05443

GranuCult^® Nutrient Broth

acc. FDA-BAM, for general cultivation of less fastidious microorganisms

• Synonym(s): Nutrient Broth, Nutrient broth
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: FDA-BAM
• Quality Level: 200
• sterility: non-sterile
• Sterilization Compatibility: autoclavable compatible
• form: granular
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 7.0 (37 °C, 8 g/L in H₂O, after autoclaving)
• solubility: 8 g/L
• bulk density: 470 kg/m³
• application(s): food and beverages; pharmaceutical
• storage temp.: 15-25°C
• suitability: heterotrophic bacteria

Description

General description

GranuCult^® prime Nutrient Broth is a general-purpose liquid media used for the isolation and growth of less fastidious microorganisms. It consists of peptone from meat, meat extract, yeast extract and sodium chloride. All these provide the basic nutrient requirements for the cultivation of non-fastidious microbes. The media can be enriched on the addition of supplements like blood.

Application

GranuCult^® Nutrient Broth is designed as a basic medium to support the growth of fastidious and non-fastidious microbes in test samples. It is an appropriate media for carrying out sub-culturing or to revive dormant strains.

Features and Benefits

• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to- batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allowing many supplements to be included, with no need to add these separately
  

Linkage

Replaces: 1.05443

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish to yellowish-brown
pH-value (25 °C): 6.6 - 7.0
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Streptococcus pyogenes ATCC 12344): ≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19118): ≤ 100
Inoculum on reference medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 100
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair to very good
Growth (Streptococcus pyogenes ATCC 12344): fair to very good
Growth (Listeria monocytogenes ATCC 19118): fair to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): fair to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): fair to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): fair to very good
Incubation: 24 ± 2 hours at 37 ± 1 °C, aerobic
Reference media: Tryptic Soy Agar

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3