- Direct visualization of newly synthesized target proteins in situ.
Direct visualization of newly synthesized target proteins in situ.
Nature methods (2015-03-17)
Susanne tom Dieck, Lisa Kochen, Cyril Hanus, Maximilian Heumüller, Ina Bartnik, Belquis Nassim-Assir, Katrin Merk, Thorsten Mosler, Sakshi Garg, Stefanie Bunse, David A Tirrell, Erin M Schuman
PMID25775042
ABSTRACT
Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.
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