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Merck

Self-Organizing 3D Human Trunk Neuromuscular Organoids.

Cell stem cell (2020-01-21)
Jorge-Miguel Faustino Martins, Cornelius Fischer, Alessia Urzi, Ramon Vidal, Severine Kunz, Pierre-Louis Ruffault, Loreen Kabuss, Iris Hube, Elisabeta Gazzerro, Carmen Birchmeier, Simone Spuler, Sascha Sauer, Mina Gouti
ABSTRACT

Neuromuscular networks assemble during early human embryonic development and are essential for the control of body movement. Previous neuromuscular junction modeling efforts using human pluripotent stem cells (hPSCs) generated either spinal cord neurons or skeletal muscles in monolayer culture. Here, we use hPSC-derived axial stem cells, the building blocks of the posterior body, to simultaneously generate spinal cord neurons and skeletal muscle cells that self-organize to generate human neuromuscular organoids (NMOs) that can be maintained in 3D for several months. Single-cell RNA-sequencing of individual organoids revealed reproducibility across experiments and enabled the tracking of the neural and mesodermal differentiation trajectories as organoids developed and matured. NMOs contain functional neuromuscular junctions supported by terminal Schwann cells. They contract and develop central pattern generator-like neuronal circuits. Finally, we successfully use NMOs to recapitulate key aspects of myasthenia gravis pathology, thus highlighting the significant potential of NMOs for modeling neuromuscular diseases in the future.

MATERIALS
Product Number
Brand
Product Description

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CNQX, ≥98% (HPLC), solid
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Gelatin from porcine skin, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture
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DL-2-Amino-5-phosphonopentanoic acid, solid
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Anti-Sox2 Antibody, Chemicon®, from rabbit
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Debrisoquine sulfate
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Anti-Myosin (Skeletal, Fast) antibody, Mouse monoclonal, clone MY-32, purified from hybridoma cell culture
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Triton X-100, laboratory grade
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N-Methyl-D-aspartic acid, ≥98% (TLC), solid
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Anti-Choline Acetyltransferase Antibody, Chemicon®, from goat
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