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  • Phospholipid-Block Copolymer Hybrid Vesicles with Lysosomal Escape Ability.

Phospholipid-Block Copolymer Hybrid Vesicles with Lysosomal Escape Ability.

Langmuir : the ACS journal of surfaces and colloids (2018-05-20)
Wei Zong, Bo Thingholm, Fabian Itel, Philipp S Schattling, Edit Brodszkij, Daniel Mayer, Steffen Stenger, Kenneth N Goldie, Xiaojun Han, Brigitte Städler
ABSTRACT

The success of nanoparticulate formulations in drug delivery depends on various aspects including their toxicity, internalization, and intracellular location. Vesicular assemblies consisting of phospholipids and amphiphilic block copolymers are an emerging platform, which combines the benefits from liposomes and polymersomes while overcoming their challenges. We report the synthesis of poly(cholesteryl methacrylate)- block-poly(2-(dimethylamino) ethyl methacrylate) (pCMA- b-pDMAEMA) block copolymers and their assembly with phospholipids into hybrid vesicles. Their geometry, their ζ-potential, and their ability to adsorb onto polymer-coated surfaces were assessed. Giant unilamellar vesicles were employed to confirm the presence of both the phospholipids and the block copolymer in the same membrane. Furthermore, the cytotoxicity of selected hybrid vesicles was determined in RAW 264.7 mouse macrophages, primary rat Kupffer cells, and human macrophages. The internalization and lysosomal escape ability of the hybrid vesicles were confirmed using RAW 264.7 mouse macrophages. Taken together, our findings illustrate that the reported hybrid vesicles are a promising complementary drug delivery platform for existing liposomes and polymersomes.

MATERIALS
Product Number
Brand
Product Description

Avanti
16:0-18:1 PC, Avanti Research - A Croda Brand
Avanti
16:0-18:1 PS (POPS), Avanti Research - A Croda Brand
Avanti
16:0-18:1 PS (POPS), Avanti Research - A Croda Brand
Avanti
16:0-18:1 PC, Avanti Research - A Croda Brand
Avanti
16:0-18:1 EPC (Cl Salt), Avanti Research - A Croda Brand 890705C
Avanti
16:0-18:1 EPC (Cl Salt), Avanti Research - A Croda Brand 890705P, powder
Avanti
14:0-06:0 NBD PC, Avanti Research - A Croda Brand 810122C
Avanti
14:0-06:0 NBD PC, Avanti Research - A Croda Brand 810122P, powder