M5017

Medium 199

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• product name: Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: L-glutamine: yes; phenol red: yes; NaHCO₃: no; sodium pyruvate: no; Earle’s salts (5% CO₂): yes; HEPES: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable