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Key Documents

GE17-5181-01

Sigma-Aldrich

SOURCE 15Q 4.6/100 PE

Synonym(s):

SOURCE 15Q Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

ligand

quaternary ammonium

material

polypropylene column

description

Ion Exchanger Type (value)

packaging

pkg of 1 ea

parameter

580 psi

bed size

4.6 mm × 100 mm

bed volume

1.7 mL

column I.D.

4.6 mm

matrix

polymeric
polystyrene/divinylbenzene

matrix active group

ammonium ion, quaternary phase

avg. part. size

15 μm

cleaning

1-14

working range

2-12

suitability

suitable for bioprocess medium

separation technique

anion exchange

General description

SOURCE 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

Features and Benefits

  • SOURCE 15Q media is highly suitable for fast, high-resolution purifications and easy scale-up
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SOURCE is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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