EIII
86072804, Human x Mouse Hybridoma hybrid, Fibroblast
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About This Item
UNSPSC Code:
41106514
product name
EIII, 86072804
biological source
Human x Mouse Hybridoma hybrid
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
pJ3.11, p79a, CFTR DNA markers
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human x mouse hybrid
Cell Line Description
This cell line may contain a sub-chromosomal fragment of human chromosome 17 bearing markers thought at the time to flank the cytotic fibrosis gene. The human DNA was donated from the human osteosarcoma cell line MNNG-HOS.
Application
Somatic cell hybrid mapping
Culture Medium
DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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