It is recommended to reconstitute this product in 20 mM sodium phosphate, pH 6.9, 0.45% NaCl, and 0.01% BSA at 5 mg/mL. Following reconstitution, store at 2 - 8°C up to 1 week. Stock solutions may be stored in working aliquots at -20°C for several months. Avoid repeated freeze/thaw cycles.
385931
Hyaluronidase, Bovine Testes
Synonym(s):
Hyaluronidase, Bovine Testes
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
conjugate
conjugate (Glucosaminoglycan)
Quality Level
form
lyophilized
specific activity
≥2500 units/mg dry wt.
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
−20°C
Related Categories
General description
Native hyaluronidase from bovine testes. Catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid.
Native hyaluronidase from bovine testes. Hyaluronic acid degrading endoglycosidase that catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid.
Note: 1 KU = 1000 units.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will cause the same turbidity reduction as 1.0 unit of International Standard preparation.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 week at 4°C.
Reconstitute in 20 mM sodium phosphate, pH 6.9, 0.45% NaCl, and 0.01% BSA.
Other Notes
Lokeshwar, V.B., et al. 2002. J. Biol. Chem.277, 33654.
Humphrey, I.H. 1957. Bull. WHO16, 291.
Humphrey, I.H. 1957. Bull. WHO16, 291.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J H HUMPHREY
Bulletin of the World Health Organization, 16(2), 291-294 (1957-01-01)
The International Standard for Hyaluronidase is derived from a blend of four batches of bovine testicular hyaluronidase which had been purified so as to contain approximately 400 units per milligram. The material was mixed with beta-lactose, and lyophilized. Tablets were
Vinata B Lokeshwar et al.
The Journal of biological chemistry, 277(37), 33654-33663 (2002-06-27)
Hyaluronidase is a hyaluronic acid-degrading endoglycosidase that is present in many toxins and the levels of which are elevated in cancer. Increased concentration of HYAL1-type hyaluronidase correlates with tumor progression and is a marker for grade (G) 2 or 3
Articles
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
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I am doing experiments on hyaluronidase activity and I would like information on how the reconstituted enzyme performs after being frozen and thawed at both -20C and -80C
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What is molecular weight of this product (in kDa)?
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The molecular weight is approximately 61 kDa. This information may be found on the Certificate of Analysis.
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We have a bottle of Hyaluronidase, Bovine Testes in our lab and need to know if it has an expiration date. The only date on the bottle is the received date written by one of the techs. If it is stored properly in the freezer what is the expiration date?
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This product is not assigned an expiration date or recommended retest date. Customers should routinely inspect products with no expiration date or recommended retest date to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information, including the lot number, so that a member of our team can reach out to you to assist further. Thank you.
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