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  • MRP1 protein expression in leukemic stem cells as a negative prognostic marker in acute myeloid leukemia patients.

MRP1 protein expression in leukemic stem cells as a negative prognostic marker in acute myeloid leukemia patients.

European journal of haematology (2017-08-15)
Maria Paprocka, Aleksandra Bielawska-Pohl, Joanna Rossowska, Agnieszka Krawczenko, Danuta Duś, Marek Kiełbiński, Olga Haus, Maria Podolak-Dawidziak, Kazimierz Kuliczkowski
ABSTRACT

It is well established that expression of multi-drug resistance (MDR) proteins (MDR1, BCRP, MDR3, MRP1, and LRP) in leukemic blasts correlates with acute myeloid leukemia (AML) patients' clinical response. Assuming that leukemic stem cells (LSC) are resistant to chemotherapy and responsible for relapse, it might be clinically relevant to evaluate the expression level of MDR proteins in LSC and relate it to the clinical outcome. Bone marrow samples from 26 patients with de novo AML were labeled with antibodies to distinguish CD34+CD38-CD123+ LSC population and with antibodies against MDR1, BCRP, MDR3, MRP1, or LRP proteins. Multicolor flow cytometry was applied to evaluate the expression of MDR proteins in blasts and LSC. Nine of 26 patients with AML attained CR (30%). High negative correlation was found between MDR1 and LRP expression in blasts and the patient's remission. MDR proteins were expressed more frequently in LSC than in leukemic blasts. High negative correlation was also observed between remission achievement and MRP1 expression in LSC. Our data present for the very first time the high negative correlation between MRP1 protein expression in LSC and AML patients' remission. It does strongly suggest that MRP1 expression in LSC is an adverse prognostic marker in patients with de novo AML.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-MDR3 Antibody, clone P3 II-26, culture supernatant, clone P3 II-26, Chemicon®
Sigma-Aldrich
Anti-MRP1 Antibody, clone QCRL-1, clone QCRL-1, Chemicon®, from mouse
Sigma-Aldrich
Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2, clone UIC2, Chemicon®, from mouse
Sigma-Aldrich
Anti-BCRP Antibody, clone BXP-34, culture supernatant, clone BXP-34, Chemicon®