ABD53
Anti-ZEB1 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
frog (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), human (immunogen homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ZEB1(6935)
Related Categories
General description
Zinc finger E-box-binding homeobox 1 (ZEB1), also known as Transcription factor 8 (TCF8) and Negative regulator of IL2 or NIL-2-A zinc finger protein is a member of the Zinc finger-Homeobox (ZFH)-family of repressors and acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. ZEB1 has also been implicated in regulating the expression of ATP1A1, E-cadherin, BCL6, and RCOR1 genes. ZEB1 regulates neurogenesis and neuronal differentiation, but is also implicated in tumor formation. ZEB1 is expressed in heart and skeletal muscle. Mutations in ZEB1 have been associated with corneal dystrophy.
Immunogen
KLH-conjugated linear peptide corresponding to human ZEB1.
Application
Anti-ZEB1 detects levels of ZEB1 proteins & has been published & validated for use in WB.
Research Category
Epigenetics & Nuclear Function
Neuroscience
Epigenetics & Nuclear Function
Neuroscience
Research Sub Category
Transcription Factors
Developmental Signaling
Transcription Factors
Developmental Signaling
Quality
Evaluated by Western Blot in mouse embryo e11 tissue lysate.
Western Blot Analysis: 1.0 µg/mL of this antibody detected ZEB1 in 10 µg of mouse embryo e11 tissue lysate.
Western Blot Analysis: 1.0 µg/mL of this antibody detected ZEB1 in 10 µg of mouse embryo e11 tissue lysate.
Target description
~200 kDa observed. The calculated molecular weight is 124 kDa, however ZEB1 has been shown at ~180-210 kDa in western blots (Dave, N., et al. (2011). J. Biol. Chem. 286(14):12024-12032).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse embryo e11 tissue lysate
Mouse embryo e11 tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Xinyao Lin et al.
Nature communications, 10(1), 2065-2065 (2019-05-08)
N6-Methyladenosine (m6A) modification has been implicated in the progression of several cancers. We reveal that during epithelial-mesenchymal transition (EMT), one important step for cancer cell metastasis, m6A modification of mRNAs increases in cancer cells. Deletion of methyltransferase-like 3 (METTL3) down-regulates
Martial Millet et al.
Cancers, 14(15) (2022-08-13)
A tumor microenvironment is characterized by its altered mechanical properties. However, most models remain unable to faithfully recreate the mechanical properties of a tumor. Engineered models based on the self-assembly method have the potential to better recapitulate the stroma architecture
Jiexin Li et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 30(4), 1578-1596 (2022-01-17)
N6-methyladenosine (m6A) methylation, which is modified by the METTL3/METTL14 complex, is a dominant internal modification in mammalian RNA and tightly linked to cancer progression. Here we reveal that METTL3-promoted cell migration, invasion, and epithelial-to-mesenchymal transition (EMT) are associated with expression
Christopher J Terranova et al.
Cell reports, 36(3), 109410-109410 (2021-07-22)
The dynamic evolution of chromatin state patterns during metastasis, their relationship with bona fide genetic drivers, and their therapeutic vulnerabilities are not completely understood. Combinatorial chromatin state profiling of 46 melanoma samples reveals an association of NRAS mutants with bivalent
Jiraporn Kantapan et al.
BMC complementary medicine and therapies, 21(1), 189-189 (2021-07-05)
Radioresistance can pose a significant obstacle to the effective treatment of breast cancers. Epithelial-mesenchymal transition (EMT) is a critical step in the acquisition of stem cell traits and radioresistance. Here, we investigated whether Maprang seed extract (MPSE), a gallotannin-rich extract
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service