AB3098
Anti-Orexin-A Antibody
Chemicon®, from rabbit
Synonym(s):
Anti-NRCLP1, Anti-OX, Anti-PPOX
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
feline, rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PPOX(5498)
Specificity
Mouse Orexin-A. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
Immunogen
An 18 amino acid peptide mapping near the amino terminus of mouse Orexin-A. The immunogen sequence is identical in human, rat, porcine, ovine, bovine and canine and has 81% homology with chicken.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
CNS Control of Metabolism
Circadian Rhythm & Sleep
CNS Control of Metabolism
Circadian Rhythm & Sleep
This Anti-Orexin-A Antibody is validated for use in ELISA, WB for the detection of Orexin-A.
Western blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 1:10,000 - 100,000 using 5-100 ng of control peptide per well. NOTE: Orexin A is a 3.5kDa protein. Adjust your SDS-PAGE gel and lane size markers accordingly.
Immunohistochemistry: Frozen 4% PFA tissue with ABC detection {Wagner et al 2000}.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: Frozen 4% PFA tissue with ABC detection {Wagner et al 2000}.
Optimal working dilutions must be determined by end user.
Physical form
Liquid in PBS, pH 7.4 with 0.1% BSA and 0.1% sodium azide.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hypothalamus-olfactory system crosstalk: orexin a immunostaining in mice.
Gascuel, J; Lemoine, A; Rigault, C; Datiche, F; Benani, A; Penicaud, L; Lopez-Mascaraque, L
Frontiers in Neuroanatomy null
Hyunjin Shin et al.
Scientific reports, 13(1), 20245-20245 (2023-11-21)
It has been suggested that stress responses induced by fasting have analgesic effects on nociception by elevating the levels of stress-related hormones, while there is limited understanding of pain control mechanisms. Here, we investigated whether acute or intermittent fasting alleviates
Organization and number of orexinergic neurons in the hypothalamus of two species of Cetartiodactyla: a comparison of giraffe (Giraffa camelopardalis) and harbour porpoise (Phocoena phocoena).
Dell, LA; Patzke, N; Bhagwandin, A; Bux, F; Fuxe, K; Barber, G; Siegel, JM; Manger, PR
Journal of Chemical Neuroanatomy null
S Schreyer et al.
Neuroscience, 164(2), 629-640 (2009-08-26)
Orexin-A, synthesized by neurons of the lateral hypothalamus helps to maintain wakefulness through excitatory projections to nuclei involved in arousal. Obvious changes in eye movements, eyelid position and pupil reactions seen in the transition to sleep led to the investigation
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