- Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Gene (2014-02-04)
Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya
PMID24487090
ABSTRACT
Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.
MATERIALS
Product Number
Brand
Product Description
Nickel, rod, 200mm, diameter 12.7mm, 99.98%
Nickel, foil, 0.5m coil, thickness 0.05mm, 99.9%
Nickel, wire reel, 10m, diameter 0.025mm, as drawn, 99.98%
Nickel, foil, 50x50mm, thickness 0.3mm, annealed, 99%
Nickel, foil, 25x25mm, thickness 0.125mm, as rolled, 99.99+%
Nickel, foil, 0.5m coil, thickness 0.15mm, annealed, 99%
Nickel, wire reel, 100m, diameter 0.5mm, as drawn, 99%
Nickel, wire reel, 0.5m, diameter 0.05mm, as drawn, 99.99+%
Nickel, foil, 2m coil, thickness 0.25mm, annealed, 99.98%
Nickel, foil, 25x25mm, thickness 3.2mm, as rolled, 99.99+%
Nickel, foil, 50x50mm, thickness 0.125mm, as rolled, 99.999%
Nickel, foil, 25x25mm, thickness 0.3mm, annealed, 99%
Nickel, foil, 0.5m coil, thickness 1.0mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.025mm, 99.9%
Nickel, foil, 0.5m coil, thickness 0.05mm, annealed, 99%
Nickel, foil, 0.3m coil, thickness 0.125mm, as rolled, 99.99+%
Nickel, foil, 50x50mm, thickness 1.0mm, as rolled, 99.99+%
Nickel, foil, 10mm disks, thickness 0.015mm, 99.9%
Nickel, tube, 500mm, outside diameter 1.0mm, inside diameter 0.8mm, wall thickness 0.1mm, hard, 99.5%
Nickel, pellets, 20g, max. size 20mm, 99.99+%
Nickel, foil, 10m coil, thickness 0.125mm, coil width 50mm, annealed, 99%
Nickel, rod, 100mm, diameter 10.0mm, 99.99+%
Nickel, foil, 0.5m coil, thickness 0.005mm, as rolled, 99.9%
Nickel, foil, 50x50mm, thickness 0.3mm, as rolled, 99.999%
Nickel, foil, 4mm disks, thickness 0.05mm, as rolled, 100%
Nickel, foil, 10mm disks, thickness 0.025mm, as rolled, 99.99+%
Nickel, foil, 25x25mm, thickness 2.0mm, annealed, 99%
Nickel, foil, 50x50mm, thickness 1.0mm, annealed, 99%
Nickel, foil, 10mm disks, thickness 0.05mm, annealed, 99%
Nickel, tube, 500mm, outside diameter 3.25mm, inside diameter 3.05mm, wall thickness 0.1mm, hard, 99.5%