Skip to Content
Merck
  • Iron oxide nanoparticles functionalized with novel hydrophobic and hydrophilic porphyrins as potential agents for photodynamic therapy.

Iron oxide nanoparticles functionalized with novel hydrophobic and hydrophilic porphyrins as potential agents for photodynamic therapy.

Journal of colloid and interface science (2015-10-12)
Oriol Penon, María J Marín, David B Amabilino, David A Russell, Lluïsa Pérez-García
ABSTRACT

The preparation of novel porphyrin derivatives and their immobilization onto iron oxide nanoparticles to build up suitable nanotools for potential use in photodynamic therapy (PDT) has been explored. To achieve this purpose, a zinc porphyrin derivative, ZnPR-COOH, has been synthesized, characterized at the molecular level and immobilized onto previously synthesized iron oxide nanoparticles covered with oleylamine. The novel nanosystem (ZnPR-IONP) has been thoroughly characterized by a variety of techniques such as UV-Vis absorption spectroscopy, fluorescence spectroscopy, X-ray photoloectron spectroscopy (XPS) and transmission electron microscopy (TEM). In order to probe the capability of the photosensitizer for PDT, the singlet oxygen production of both ZnPR-IONP and the free ligand ZnPR-COOH have been quantified by measuring the decay in absorption of the anthracene derivative 9,10-anthracenedipropionic acid (ADPA), showing an important increase on singlet oxygen production when the porphyrin is incorporated onto the IONP (ZnPR-IONP). On the other hand, the porphyrin derivative PR-TRIS3OH, incorporating several polar groups (TRIS), was synthesized and immobilized with the intention of obtaining water soluble nanosystems (PR-TRIS-IONP). When the singlet oxygen production ability was evaluated, the values obtained were similar to ZnPR-COOH/ZnPR-IONP, again much higher in the case of the nanoparticles PR-TRIS-IONP, with more than a twofold increase. The efficient singlet oxygen production of PR-TRIS-IONP together with their water solubility, points to the great promise that these new nanotools represent for PDT.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Carbon, mesoporous
Sigma-Aldrich
Carbon, mesoporous, nanopowder, less than 500 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
Sigma-Aldrich
Carbon, mesoporous, less than 100 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
Sigma-Aldrich
Carbon, mesoporous, nanopowder, graphitized, less than 250 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
Sigma-Aldrich
Carbon, mesoporous, hydrophilic pore surface
Sigma-Aldrich
Sodium bicarbonate, BioXtra, 99.5-100.5%
Sigma-Aldrich
Sodium bicarbonate, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Pyrrole, ≥98%, FCC, FG
Sigma-Aldrich
Pyrrole, reagent grade, 98%
Sigma-Aldrich
Ethyl 7-bromoheptanoate, 97%
Sigma-Aldrich
Methane, electronic grade, ≥99.998%
Sigma-Aldrich
Sodium bicarbonate, ReagentPlus®, ≥99.5%, powder
Sigma-Aldrich
Activated Charcoal Norit®, Norit® RB3, for gas purification, steam activated, rod
Sigma-Aldrich
Activated Charcoal Norit®, Norit® PK 1-3, from peat, steam activated, granular
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX ultra, from peat, corresponds U.S. Food chemicals codex (3rd Ed.), steam activated and acid washed, highly purified, powder
Sigma-Aldrich
Sodium bicarbonate, ACS reagent, ≥99.7%
Sigma-Aldrich
Sodium bicarbonate, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E500, 99.0-100.5%, powder
Sigma-Aldrich
Sodium bicarbonate, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
Sigma-Aldrich
Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
Sigma-Aldrich
Sodium bicarbonate, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.7%
Sigma-Aldrich
Activated Charcoal Norit®, Norit® CA1, wood, chemically activated, powder
Sigma-Aldrich
Sodium hydrogencarbonate, −40-+140 mesh, ≥95%
Sigma-Aldrich
Potassium hydroxide solution, 0.1 M in ethanol
Sigma-Aldrich
Potassium hydroxide solution, 0.1 M in isopropanol
Sigma-Aldrich
Potassium hydroxide solution, 1 M
Sigma-Aldrich
Potassium hydroxide solution, 0.5 M
Sigma-Aldrich
Diphenyl ether, SAJ first grade, ≥98.0%
Sigma-Aldrich
Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm
Sigma-Aldrich
Carbon nanofibers, pyrolitically stripped, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm