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G005902

HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express OH-5, 15 cm x 4.6 mm, I.D., 2.7 μm particles (53778-U)
mobile phase: [A] 0.005 M ammonium formate, pH 3.15 with concentrated TFA [B] acetonitrile (30:70, A:B)
flow rate: 0.5 mL/min
pressure: 841 psi (58 bar)
column temp.: 35 °C
detector: ELSD (detector other: Nebulizer 60º C, Evaporator 80º C, Gas flow 1.60 SLM (Standard Liters/ Minute), LED 100%, Smoothing 5.0 S, PMT ( Photomultiplier) Gain 1.0)
injection: 10 μL
sample: 0.5 mg/mL each prepared in 50:50 Water:Acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application shows the analysis of sugars by HILIC Chromatography with ELSD detection. The purpose of the work shown here was to develop a HILIC method that could quickly separate mono- and di-saccharides with good sensitivity and reproducibility utilizing the Ascentis Express OH-5 column and an Evaporative Light Scattering Detecctor (ELSD) detector.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material
    Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material
    Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material

analytical column

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