Please follow this link to access the product information sheet featuring the protocol: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/483/sca20bul.pdf
SCA20
Sucrose Assay Kit
sufficient for 20 assays
Synonym(s):
Sucrose Test Kit
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About This Item
Pricing and availability is not currently available.
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usage
sufficient for 20 assays
Quality Level
analyte chemical class(es)
sugars (sucrose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
General description
Application
This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.
Kit Components Only
Product No.
Description
- glucose assay reagent 50 mL
- sucrose assay reagent 1 mL/vial
- sucrose standard 500 mg
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
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