S0266

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

• Synonym(s): 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
• Linear Formula: NH₂(CH₂)₃NH(CH₂)₄NH₂
• CAS Number: 124-20-9
• Molecular Weight: 145.25
• Beilstein: 1698591
• EC Number: 204-689-0
• MDL number: MFCD00008229
• UNSPSC Code: 12352200
• PubChem Substance ID: 24899434
• NACRES: NA.52

Properties

• grade: for molecular biology
• product line: BioReagent
• Assay: ≥98%
• form: solid or liquid
• mol wt: 145.25 (AMU)
• technique(s): cell culture | mammalian: suitable
• refractive index: n20/D 1.479 (lit.)
• density: 0.925 g/mL at 25 °C (lit.)
• foreign activity: DNase, RNase, and protease, none detected
• storage temp.: room temp
• SMILES string: NCCCCNCCCN
• InChI: 1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
• InChI key: ATHGHQPFGPMSJY-UHFFFAOYSA-N
• Gene Information: rat ... Ppm1a(24666)

Description

General description

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Application

Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Caution

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): 233.6 °F - closed cup
• Flash Point(C): 112 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves