G4134

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• Synonym(s): G-6-P-DH, Zwischenferment
• CAS Number: 9001-40-5
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• EC Number: 232-602-6
• MDL number: MFCD00081656
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type IX
• Quality Level: 200
• form: lyophilized powder
• specific activity: 200-400 units/mg protein (modified Warburg-Christian)
• mol wt: 128 kDa
• β-NADP and β-NADPH content: ≤10 mmol/mol
• application(s): agriculture
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.[1]

Application

Glucose-6-phosphate dehydrogenase is used:

• To test ketose reductase activity in developing maize endosperm.[2]
• For recycling microassay of β-NADP and β-NADPH.[3][4]
• To measure the intracellular levels of NADPH and total NADP.[5]
• To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.[6]

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide.[7] Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death.[8] Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.[9]

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Physical form

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves