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MAB1598

Sigma-Aldrich

Anti-Post Synaptic Density Protein 95 Antibody, clone 7E3-1B8

clone 7E3-1B8, Chemicon®, from mouse

Synonym(s):

PSD-95

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7E3-1B8, monoclonal

species reactivity

bovine, rat, mouse

species reactivity (predicted by homology)

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Dlg4(100137840)
human ... DLG4(1742)
mouse ... Dlg4(13385)
rat ... Dlg4(29495)

General description

The postsynaptic density (PSD) is an electron dense structure just beneath the postsynaptic membrane. Several functions have been proposed for the PSD including regulating receptor number and clustering, anchoring signal transduction molecules at the synapse and mediating adhesion between the presynaptic and postsynaptic membranes. The most abundant PSD protein is the [alpha] subunit of the type II calcium/calmodulin dependent protein kinase ([alpha]CaMKII). This protein is likely to play a role in the calcium-mediated signal transduction at the synapse that mediates certain forms of synaptic plasticity. Another major PSD protein is PSD-95, a member of the guanylate kinase family (GUK) of proteins.

Specificity

Reacts with recombinant rat PSD-95 and identifies a band at approximately ~100 kDa, corresponding to PSD-95, on western blots of rat brain, mouse brain and bovine brain extracts. Also present in rat brain and mouse brain tissue extracts are bands of approximately 80 kDa and 50 kDa. PSD-95 is believed to initiate NMDA receptor clustering on the post synaptic membrane.

Immunogen

Recombinant rat PSD-95

Application

Anti-Post Synaptic Density Protein 95 Antibody, clone 7E3-1B8 is an antibody against Post Synaptic Density Protein 95 for use in IC, IP & WB.
Immunocytochemistry:
A previous lot of this antibody was used in IC.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by the end user.

Quality

Evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected PSD-95 on 10 µg of Mouse brain lysates.

Target description

~ 95 kDa

Linkage

Replaces: 04-1066

Physical form

Format: Purified
Purified mouse monoclonal IgG1 liquid in buffer containing PBS, pH 7.2, with 0.09% sodium azide and 50% glycerol.

Analysis Note

Control
Mouse brain, rat brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Activity-dependent local translation of matrix metalloproteinase-9.
Dziembowska, M; Milek, J; Janusz, A; Rejmak, E; Romanowska, E; Gorkiewicz, T; Tiron et al.
The Journal of Neuroscience null
PSD-95 is post-transcriptionally repressed during early neural development by PTBP1 and PTBP2.
Zheng, S; Gray, EE; Chawla, G; Porse, BT; O'Dell, TJ; Black, DL
Nature Neuroscience null
Brain-derived neurotrophic factor restores synaptic plasticity in a knock-in mouse model of Huntington's disease.
Lynch, G; Kramar, EA; Rex, CS; Jia, Y; Chappas, D; Gall, CM; Simmons, DA
The Journal of Neuroscience null
Kv4.2 potassium channels segregate to extrasynaptic domains and influence intrasynaptic NMDA receptor NR2B subunit expression.
Kaufmann, WA; Matsui, K; Jeromin, A; Nerbonne, JM; Ferraguti, F
Brain Structure &Amp; Function null
Jennifer D Cooke et al.
Psychopharmacology, 231(23), 4429-4441 (2014-05-02)
Monoamine reuptake inhibitors can stimulate expression of brain-derived neurotrophic factor (BDNF) and alter long-term potentiation (LTP), a widely used model for the synaptic mechanisms that underlie memory formation. BDNF expression is upregulated during LTP, and BDNF in turn positively modulates

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