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MAB10005

Sigma-Aldrich

Anti-ABCA1 Antibody, clone AB.H10

clone AB.H10, Chemicon®, from mouse

Synonym(s):

ATP-binding cassette transporter 1, ABC-1, Cholesterol efflux regulatory protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

AB.H10, monoclonal

species reactivity

human, chicken, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ABCA1(19)

General description

Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Specificity

Recognize specifically ABCA1 from human, mouse, and chicken.

Immunogen

Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.

Application

ELISA
Immunohistochemistry (Paraffin): 5 μg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required.
Immunoprecipitation
Western Blot: 1 μg/mL. Predicted molecular weight: 254 kDa.

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
This Anti-ABCA1 Antibody, clone AB.H10 is validated for use in ELISA, IP, WB, IH(P) for the detection of ABCA1.

Physical form

Format: Purified
Presented as a liquid in PBS, pH 7.4 with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Raimund Oberle et al.
Life science alliance, 5(9) (2022-05-17)
Despite enormous efforts to improve therapeutic options, pancreatic cancer remains a fatal disease and is expected to become the second leading cause of cancer-related deaths in the next decade. Previous research identified lipid metabolic pathways to be highly enriched in
Sebastian Zimmer et al.
Science translational medicine, 8(333), 333ra50-333ra50 (2016-04-08)
Atherosclerosis is an inflammatory disease linked to elevated blood cholesterol concentrations. Despite ongoing advances in the prevention and treatment of atherosclerosis, cardiovascular disease remains the leading cause of death worldwide. Continuous retention of apolipoprotein B-containing lipoproteins in the subendothelial space
Decreased APOE-containing HDL subfractions and cholesterol efflux capacity of serum in mice lacking Pcsk9.
Choi, S; Aljakna, A; Srivastava, U; Peterson, BR; Deng, B; Prat, A; Korstanje, R
Lipids in Health and Disease null
Patrick He et al.
Pharmacological reports : PR, 71(6), 1219-1226 (2019-11-02)
The pathogenesis of chronic obstructive pulmonary disease (COPD) is associated with dyslipidemia, an established co-morbidity. Statins treat hypercholesterolemia, but more recently have been trailed in the setting of COPD for their potential anti-inflammatory benefits. The outcomes of prospective trials however
Hong Y Choi et al.
Frontiers in pharmacology, 12, 679456-679456 (2021-06-08)
Objective: Our recent studies showed that desmocollin 1 (DSC1) binds to apoA-I in order to inhibit apoA-I-mediated high density lipoprotein (HDL) biogenesis in atherosclerotic plaques. To promote HDL biogenesis in the plaque, here we search for small molecules that block

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