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05-719

Sigma-Aldrich

Anti-Phosphatidylserine Antibody, clone 1H6

clone 1H6, Upstate®, from mouse

Synonym(s):

Anti-Phosphatidylserine, Clone 1H6 Anti-Phosphatidylserine, Phosphatidylserine Detection

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable

isotype

IgG

shipped in

dry ice

target post-translational modification

phosphorylation (pSer)

Gene Information

human ... PTDSS1(9791)

General description

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Phosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.

Specificity

Recognizes phosphatidylserine (PS) in cell membranes

Immunogen

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol

Application

Anti-Phosphatidylserine Antibody, clone 1H6 is an antibody against Phosphatidylserine for use in FC, IH.
Flow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

routinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)

Target description

none given

Physical form

Format: Purified
Protein G Purified
Purified IgG supplied in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 before the addition of glycerol to 30%. Liquid at -20°C.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Estela M Galván et al.
Infection and immunity, 75(3), 1272-1279 (2006-12-21)
The pH 6 antigen (Psa) of Yersinia pestis consists of fimbriae with adhesive properties of potential importance for the pathogenesis of plague, including pneumonic plague. The Psa fimbriae mediate bacterial binding to human alveolar epithelial cells. The Psa fimbriae bound
Ibtehaj Naqvi et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 26(4), 1020-1031 (2018-03-20)
Nucleic acid binding polymers (NABPs) have been extensively used as vehicles for DNA and RNA delivery. More recently, we discovered that a subset of these NABPs can also serve as anti-inflammatory agents by capturing pro-inflammatory extracellular nucleic acids and associated protein
S Grunewald et al.
International journal of andrology, 31(3), 325-330 (2007-06-19)
Human sperm have been documented to display apoptosis-like features such as externalization of phosphatidylserine (EPS), disruption of the transmembrane mitochondrial potential (MMP) and activation of caspases. Our aim was to evaluate possible association between activation of the apoptosis cascade in
Manjarika De et al.
Molecular therapy. Nucleic acids, 10, 9-27 (2018-03-04)
There is a pressing need for a ubiquitously expressed antigen or receptor on the tumor surface for successful mitigation of the deleterious side effects of chemotherapy. Phosphatidylserine (PS), normally constrained to the intracellular surface, is exposed on the external surface
Apoptosis: giving phosphatidylserine recognition an assist--with a twist.
Fadok, Valerie A and Henson, Peter M
Current Biology, 13, R655-R657 (2003)

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