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UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell A160 Peptide C18 Using Ammonium Formate as a Mobile Phase Modifier

UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell A160 Peptide C18 Using Ammonium Formate as a Mobile Phase Modifier application for UHPLC

Materiais

coluna analítica

Nº do produto
Descrição
Preços

BIOshell A160 Peptide C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25 (8 mM ammonium formate, pH 2.6 with formic acid:acetonitrile); [B] 50:50 (8 mM ammonium formate, pH 2.6 with formic acid:acetonitrile); (77:23, A:B)

flow rate

0.2 mL/min

column temp.

75 °C

detector

MS, ESI(+), TIC 100-3000 m/z

injection

0.5 μL

sample

Mixture of six insulin variants, 100 μg/mL, 8 mM ammonium formate, pH 2.6 with formic acid

Descrição

Nota de análise

BIOshell A160 Peptide C18 is an ideal column for reversed-phase separations of small proteins to large peptide sequences. There is over 75% sequence similarity between all six of the insulin variants in the sample; therefore, separation of these variants is achieved through the subtle differences in the polarities of surface residues on the peptides. The use of ammonium formate as a mobile phase modifier yields more efficient peaks and less signal suppression during electrospray ionization (ESI) than some traditional ion-pair reagents (trifluoroacetic acid, formic acid).

Informações legais

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