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Merck

Studies on inorganic pyrophosphatase using imidodiphosphate as a substrate.

FEBS letters (1986-09-29)
I N Smirnova, A A Baykov, S M Avaeva
RESUMO

Baker's yeast inorganic pyrophosphatase has been found to catalyze Mg2+-dependent hydrolysis of imidodiphosphate yielding phosphate and amidophosphate. The reaction proceeds linearly in the presteady state. The catalytic constant is maximal at pH 9.0 and equals 0.5 min-1. Kinetic titrations of the enzyme with imidodiphosphate and Mg2+ have provided direct evidence for the involvement of three Mg2+ per active site in the transition state of the pyrophosphatase reaction.

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Sigma-Aldrich
Imidodiphosphate sodium salt, ≥97%