Pular para o conteúdo
MilliporeSigma
  • Mathematical model of the binding of allosteric effectors to the Escherichia coli PII signal transduction protein GlnB.

Mathematical model of the binding of allosteric effectors to the Escherichia coli PII signal transduction protein GlnB.

Biochemistry (2013-03-23)
Ricardo Alves da Rocha, Thiago André Weschenfelder, Fernanda de Castilhos, Emanuel Maltempi de Souza, Luciano Fernandes Huergo, David Alexander Mitchell
RESUMO

PII proteins are important regulators of nitrogen metabolism in a wide variety of organisms: the binding of the allosteric effectors ATP, ADP, and 2-oxoglutarate (2-OG) to PII proteins affects their ability to interact with target proteins. We modeled the simultaneous binding of ATP, ADP, and 2-OG to one PII protein, namely GlnB of Escherichia coli, using a modeling approach that allows the prediction of the proportions of individual binding states. Four models with different binding rules were compared. We selected one of these models (that assumes that the binding of the first nucleotide to GlnB makes it harder for subsequent nucleotides to bind) and used it to explore how physiological concentrations of ATP, ADP, and 2-OG would affect the proportions of those states of GlnB that interact with the target proteins ATase and NtrB. Our simulations indicate that GlnB can, as suggested by previous researchers, act as a sensor of both 2-OG and the ATP:ADP ratio. We conclude that our modeling approach will be an important tool in future studies concerning the PII binding states and their interactions with target proteins.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
α-Ketoglutaric acid, BioReagent, suitable for cell culture, suitable for insect cell culture
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
α-Ketoglutaric acid potassium salt, ≥98% (enzymatic)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
α-Ketoglutaric acid disodium salt dihydrate, ≥98.0% (dried material, NT)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
α-Ketoglutaric acid, ≥98.5% (NaOH, titration)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
α-Ketoglutaric acid sodium salt, ≥98% (titration)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
Adenosine 5′-diphosphate, ≥95% (HPLC)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
α-Ketoglutaric acid, 99.0-101.0% (T)
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Sigma-Aldrich
α-Ketoglutaric acid sodium salt, BioUltra
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade
Supelco
α-Ketoglutaric acid disodium salt hydrate, ≥95%
SKU
Tamanho da embalagem
Disponibilidade
Preço
Quantidade