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  • Effects of chlortetracycline amended feed on anaerobic sequencing batch reactor performance of swine manure digestion.

Effects of chlortetracycline amended feed on anaerobic sequencing batch reactor performance of swine manure digestion.

Bioresource technology (2012-10-02)
Teal M Dreher, Henry V Mott, Christopher D Lupo, Aaron S Oswald, Sharon A Clay, James J Stone
RESUMO

The effects of antimicrobial chlortetracycline (CTC) on the anaerobic digestion (AD) of swine manure slurry using anaerobic sequencing batch reactors (ASBRs) was investigated. Reactors were loaded with manure collected from pigs receiving CTC and no-antimicrobial amended diets at 2.5 g/L/d. The slurry was intermittently fed to four 9.5L lab-scale anaerobic sequencing batch reactors, two with no-antimicrobial manure, and two with CTC-amended manure, and four 28 day ASBR cycles were completed. The CTC concentration within the manure was 2 8 mg/L immediately after collection and 1.02 mg/L after dilution and 250 days of storage. CTC did not inhibit ASBR biogas production extent, however the volumetric composition of methane was significantly less (approximately 13% and 15% for cycles 1 and 2, respectively) than the no-antimicrobial through 56 d. CTC decreased soluble chemical oxygen demand and acetic acid utilization through 56 d, after which acclimation to CTC was apparent for the duration of the experiment.

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Supelco
Chlortetracycline hydrochloride, VETRANAL®, analytical standard
Sigma-Aldrich
Chlortetracycline hydrochloride, ≥91.0% dry basis (HPLC)
Sigma-Aldrich
Chlortetracycline hydrochloride, suitable for fluorescence, BioReagent, from Streptomyces aureofaciens, ≥85.0% (HPLC)
Millipore
Chlortetracycline Selective Supplement, suitable for microbiology