Pular para o conteúdo
Merck
  • Production of (R)-3-hydroxybutyric acid by fermentation and bioconversion processes with Azohydromonas lata.

Production of (R)-3-hydroxybutyric acid by fermentation and bioconversion processes with Azohydromonas lata.

Bioresource technology (2011-04-22)
Charles U Ugwu, Yutaka Tokiwa, Toshio Ichiba
RESUMO

Feasibility of producing (R)-3-hydroxybutyric acid ((R)-3-HB) using wild type Azohydromonas lata and its mutants (derived by UV mutation) was investigated. A. lata mutant (M5) produced 780 m g/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (R)-3-HB. (R)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (R,S)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (R,S)-1,3-butanediol (3% v/v), resulted in (R)-3-HB concentrations of 6.5 g/l, 7.3g/l and 8.7 g/l, respectively.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
(R)-(−)-1,3-Butanediol, 95%
Sigma-Aldrich
(±)-1,3-Butanediol, ReagentPlus®, 99.5%
Sigma-Aldrich
(±)-1,3-Butanediol, anhydrous, ≥99%