One suggestion is to use PBS with 0.05% Tween 20. Extraction with urea/SDS is not suggested because this is incompatible with the antibodies in the Seppro products.
About This Item
Produtos recomendados
coluna P × C
6.4 mm × 63 mm
capacidade
1 mg total protein loading(D-Ribulose 1,5-Diphosphate Carboxylase (Rubisco))
temperatura de armazenamento
2-8°C
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Descrição geral
Aplicação
Informações legais
Código de classe de armazenamento
10 - Combustible liquids
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How can I extract my plant proteins for use with Seppro® Rubisco kit, Product SEP080?
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I originally purchased my Seppro RuBisCO LC2 column from Genway. Can I still contact Genway for technical support?
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All inquiries on the Seppro technology are now to be referred to Sigma-Aldrich. Customers should not contact Genway for technical assistance.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I do TCA/acetone precipitation for isolating plant proteins to use with the Seppro® Rubisco kit, Product SEP080?
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TCA/acetone precipitation is problematic because it may be difficult to redissolve the protein pellet afterwards. The following paper provides some tips for extraction: Cellar, N.A., et al., J. Chromatography B, 861, 29-39 (2008).
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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