R2631
Pvu II from Proteus vulgaris
Restriction Enzyme
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
Produtos recomendados
grau
for molecular biology
forma
buffered aqueous glycerol solution
concentração
10,000 units/mL
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Especificidade
Recognition sequence: 5′-CAG/CTG-3′
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
Aplicação
PvuII is a DNA restriction enzyme used for molecular biology methods to cleave the recognition site 5′-CAG/CTG-3′ to generate DNA fragments with blunt termini.
Outras notas
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Comment: This enzyme exhibits star activity when used under conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH >8.0.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
Pvu II does not cut CAGm4CTG or CAGm5CTG.
forma física
Solution in 20 mM Tris-HCl, pH 7.7, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C
produto relacionado
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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T R Gingeras et al.
Nucleic acids research, 9(18), 4525-4536 (1981-09-25)
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5'
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Epigenetics, 7(12), 1368-1378 (2012-10-19)
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