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P9290

Sigma-Aldrich

Proteinase K–Agarose from Tritirachium album

lyophilized powder

Sinônimo(s):

Proteinase K-Agarose

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About This Item

Número MDL:
Código UNSPSC:
41106500
NACRES:
NA.56

fonte biológica

microbial (T.album
T. ALBUM)

forma

lyophilized powder

Extensão da rotulagem

30-140 units per g solid

técnica(s)

DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable

inchaço

1 g swells to ~5 mL

temperatura de armazenamento

−20°C

Aplicação

Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.

Ações bioquímicas/fisiológicas

The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.

Características e benefícios

• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.

Definição da unidade

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

forma física

Lyophilized powder stabilized with lactose

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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