L7293
Monoclonal Anti-LSD1 (AOF2) antibody produced in mouse
~2 mg/mL, clone LSD1-12, purified immunoglobulin, buffered aqueous solution
Sinônimo(s):
Anti-Amine oxidase, flavin-containing, 2, Anti-BHC110, Anti-BRAF-HDAC complex protein 110, Anti-KIAA0601 protein, Anti-Lysine-specific demethylase 1
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About This Item
Produtos recomendados
fonte biológica
mouse
conjugado
unconjugated
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
LSD1-12, monoclonal
forma
buffered aqueous solution
peso molecular
antigen ~110 kDa
reatividade de espécies
mouse, human
concentração
~2 mg/mL
técnica(s)
western blot: 0.5-1 μg/mL using MCF7 total cell extract
Isotipo
IgG1
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... AOF2(23028)
mouse ... Aof2(99982)
Descrição geral
Monoclonal Anti-LSD1 (AOF2) (mouse IgG1 isotype) is derived from the hybridoma LSD1-12 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Lysine specific demethylase 1 (LSD1) also known as amine oxidase flavin-containing domain 2 (AOF2) () is a lysine-specific demethylase. It is a nuclear protein containing a SWIRM (Swi3p, Rsc8p and Moira) domain, a flavin adenine dinucleotide (FAD)-binding motif and an amine oxidase domain. The LSD1 gene is mapped to human chromosome 1p36.12. LSD1 is referred to by additional names including KDM1A protein, BHC110(BRAF-HDAC complex protein 110) and nuclear polyamine oxidase (NPAO)().
Especificidade
Monoclonal Anti-LSD1 (AOF2) recognizes human and mouse LSD1.
Imunogênio
synthetic peptide corresponding to amino acids 832-847 of human LSD1/BHC110. This sequence is identical in bovine, rat, mouse, and frog.
Aplicação
Monoclonal Anti-LSD1 (AOF2) antibody produced in mouse has been used:
- immunofluorescence
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Ações bioquímicas/fisiológicas
Lysine Specific Demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription.LSD1 action can signal either activation or repression of transcription. Demethylation of histone H3 Lys4 by LSD1 leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to depression of androgen receptor target genes. The activity of LSD1 (AOF2) is regulated by its associated factors namely, corepressor to the REST (RE1 silencing transcription factor/neural restrictive silencing factor)(CoREST). This, in turn, promotes demethylation by enhancing the association of LSD1 with the nucleosomes and also protects LSD1 from proteasomal degradation. LSD1 is a component of nucleosome remodeling and deacetylase (NuRD). It regulates neuronal development and favors neural stem cell proliferation. It highly expressed in acute myeloid leukemia and many solid tumors.
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Armazenamento e estabilidade
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
The cancer driver genes IDH1 2, JARID1C KDM5C, and UTX KDM6A: crosstalk between histone demethylation and hypoxic reprogramming in cancer metabolism
Chang S, et al.
Experimental & Molecular Medicine, 51(6), 1-17 (2019)
Jing Wang et al.
Nature genetics, 41(1), 125-129 (2008-12-23)
Histone methylation and DNA methylation cooperatively regulate chromatin structure and gene activity. How these two systems coordinate with each other remains unclear. Here we study the biological function of lysine-specific demethylase 1 (LSD1, also known as KDM1 and AOF2), which
Alba Maiques-Diaz et al.
Epigenomics, 8(8), 1103-1116 (2016-08-02)
LSD1 (KDM1A; BHC110; AOF2) was the first protein reported to exhibit histone demethylase activity and has since been shown to have multiple essential roles in mammalian biology. Given its enzymatic activity and its high-level expression in many human malignancies, a
Daniela Magliulo et al.
Frontiers in oncology, 8, 255-255 (2018-08-04)
Acute myeloid leukemia (AML) is a heterogeneous hematopoietic malignancy characterized by the accumulation of incompletely differentiated progenitor cells (blasts) in the bone marrow and blood, and by suppression of normal hematopoiesis. It has recently become apparent that the AML genome
Yujiang Shi et al.
Cell, 119(7), 941-953 (2004-12-29)
Posttranslational modifications of histone N-terminal tails impact chromatin structure and gene transcription. While the extent of histone acetylation is determined by both acetyltransferases and deacetylases, it has been unclear whether histone methylation is also regulated by enzymes with opposing activities.
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