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G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Sinônimo(s):

blocking buffer

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About This Item

Código UNSPSC:
41105300
NACRES:
NA.25

grau

for molecular biology

forma

powder blend

temperatura de armazenamento

room temp

Categorias relacionadas

Descrição geral

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Aplicação

Suitable for use as a non-specific blocking agent for Western blots.

Reconstituição

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

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Nº do produto
Descrição
Preços

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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Protocolos

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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