UT-1

87100701, hamster ovary, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: UT-1, 87100701
• fonte biológica: hamster ovary
• modo de crescimento: Adherent
• cariótipo: 2n = 22
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Hamster Chinese ovary

Descrição de linhagem celular

Derived from parent line CHO-K1. Cells were exposed to increasing concentrations of compactin over 8 months. During this exposure the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase synthesis increased 500-fold. The enzyme controls cholesterol synthesis.

Aplicação

Enzymatic studies

Meio de cultura

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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