AC2

86061902, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propriedades

• product name: AC2, 86061902
• fonte biológica: mouse blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Interleukin 3 (IL-3)
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse lymphoblast

Descrição de linhagem celular

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

Aplicação

Growth factor studies, IL-2 assays

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO₂; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

Outras notas

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