HeLa9903

11033105, human cervix, Epithelial

• Sinônimo(s): HeLa
• Código UNSPSC: 41106514

Propriedades

• product name: HeLa9903, 11033105, from human cervix
• fonte biológica: human cervix
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

HeLa, genetic modification, the hER alpha expression construct, Oestrogen-Responsive Element

Descrição de linhagem celular

HeLa9903 is a HeLa cell line stably transfected with two constructs: the hER alpha expression construct; a firefly luciferase reporter construct bearing Oestrogen-Responsive Element (ERE) driven by a mouse metallothionein (MT) promoter TATA element. HeLa9903 can be used to test for evaluation of chemicals based on OECD Test Guideline: The stably transformed human oestrogen receptor-alpha transcriptional; activation assay for detection of oestrogenic activity of chemicals.

Perfil do DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Meio de cultura

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 16μg/ml Blasticidin S HCL + 800μg/ml G418.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Informações legais

US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.