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C2978

Sigma-Aldrich

CelLytic™ M

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

Sinônimo(s):

Whole-cell protein extraction solution

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About This Item

Código UNSPSC:
41116134
NACRES:
NA.56

Nível de qualidade

200

Formulário

solution

temperatura de armazenamento

room temp

Descrição geral

CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.

Características e benefícios

  • Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
  • Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
  • Convenient: Ready-to-use reagent requires no scraping from culture plates
  • Fast: Rapid cell lysis at room temperature.

Quantidade

CelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).

Nota de análise

Use 125 μl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 μL for a 100 mm plate; 200-400 μL for a 35 mm plate.

Informações legais

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000375981
0000359855
0000358467
0000358467
0000359855

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Filipe Cabreiro et al.
Free radical biology & medicine, 51(8), 1575-1582 (2011-08-16)
The superoxide free radical (O(2)(•-)) has been viewed as a likely major contributor to aging. If this is correct, then superoxide dismutase (SOD), which removes O(2)(•-), should contribute to longevity assurance. In Caenorhabditis elegans, overexpression (OE) of the major cytosolic
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Journal of gastroenterology and hepatology, 31(10), 1717-1726 (2016-10-30)
The aim of this study was to compare HOXB7 expression level between gastric cancer and non-cancerous gastric tissues. Additionally, the functional effects of HOXB7, including its pro-migration or invasion and anti-apoptosis roles, were evaluated in gastric cancer cells. Both gene
Vinay Kumar Tripathi et al.
Molecular neurobiology, 54(5), 3633-3651 (2016-05-22)
Expression of various cytochrome P450s (CYPs) in mammalian brain cells is well documented. However, such studies are hampered in neural/glial cells of human origin due to nonavailability of human brain cells. To address this issue, we investigated the expression and
Yanyong Kang et al.
Nature, 558(7711), 553-558 (2018-06-15)
G-protein-coupled receptors comprise the largest family of mammalian transmembrane receptors. They mediate numerous cellular pathways by coupling with downstream signalling transducers, including the hetrotrimeric G proteins Gs (stimulatory) and Gi (inhibitory) and several arrestin proteins. The structural mechanisms that define
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Nonalcoholic fatty liver disease (NAFLD) is a major health problem worldwide, and is often associated with lipotoxic injury, defective mitochondrial function, and insulin resistance. Thyroid hormones (THs) are important regulators of hepatic lipid metabolism. Among the THs, diiodothyronine (T
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