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93595

Sigma-Aldrich

Azul de triptano

0.4%, for microscopy

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About This Item

Fórmula empírica (Notação de Hill):
C34H24N6Na4O14S4
Número CAS:
72-57-1
Peso molecular:
960.81
Beilstein:
4360496
Número MDL:
MFCD00003969
Código UNSPSC:
12171500
ID de substância PubChem:
329770254
NACRES:
MA.02

grau

for microscopy

Nível de qualidade

200

forma

liquid

prazo de validade

limited shelf life, expiry date on the label

concentração

0.4%

cor

blue to very dark blue

densidade

1.007 g/mL at 20 °C

εmax

1.4 at 603 nm in methanol

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

chave InChI

GLNADSQYFUSGOU-UHFFFAOYSA-J

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Descrição geral

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Aplicação

Trypan blue applications include:
  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes
Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Ações bioquímicas/fisiológicas

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

forma física

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H350

Classificações de perigo

Carc. 1B

Código de classe de armazenamento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificados de análise (COA)

Lot/Batch Number
BCCL2819
BCCL5345
BCCK8639
BCCK2372
BCCJ9155

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