MABF856

Anti-Helios Antibody, clone 22F6

clone 22F6, from hamster(Armenian)

• Sinônimo(s): Zinc finger protein Helios, Ikaros family zinc finger protein 2, Helios
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: hamster (Armenian)
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 22F6, monoclonal
• reatividade de espécies: human, mouse
• técnica(s): flow cytometry: suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_057344
• nº de adesão UniProt: Q9UKS7
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... IKZF2(22807)

Descrição

Descrição geral

Zinc finger protein Helios (Uniprot Q9UKS7; also known as Ikaros family zinc finger protein 2, Zinc finger DNA binding protein Helios, Zinc finger protein subfamily 1A member 2) is encoded by the IKZF2 (also known as ANF1A2, HELIOS, ZNF1A2, ZNFN1A2) gene (Gene ID 22807) in human. Helios, Ikaros, Aiolos, Eos, and Pegasus constitute a family of transcription factors characterized by six highly conserved C2H2 zinc fingers. Their four N-terminal zinc fingers mediate DNA binding, while the two C-terminal zinc fingers are responsible for homodimeric and heterodimeric protein interactions. Eos and Pegasus are expressed in a wide variety of tissues, whereas Helios, Ikaros, and Aiolos are limited to the hematopoietic system. Specifically, Helios expression is reported to be restricted to a subpopulation (approximately 70%) of Foxp3(+) T cells in mice and humans.

Especificidade

Expected to react with isoform 1, 2 (Helios 1v), 3 (Helios del(Ex3,4)), 4 (Helios del(Ex6)v), 5 (Helios 1+2a,2b, Helios 1v+2a, Helios del(Ex3)+2a), 6 (Helios 1+5a, Helios 1+5a,5b), 7 (Helios del(Ex5)v), but not isoform 8 (Helios S).

Imunogênio

Epitope: N-terminal

Linear peptide corresponding to a human Helios N-terminal sequence.

Aplicação

Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Helios in 1X10E6 human Jurkat cells.
Flow Cytometry Analysis: A representative lot detected Helios immunoreactivity among the majority of CXCR5-positive cells within the CD4+Foxp3+ population of murine peripheral lymph node (LN) cells (Chung, Y., et al. (2011). Nat Med.17(8):983-988).
Flow Cytometry Analysis: A representative lot detected Helios immunoreactivity in the Foxp3-positive, but not Foxp3-negative, subpopulation of CD4+ cells from murine peripheral lymph node (LN) and human PBMCs (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Flow Cytometry Analysis: A representative lot revealed the age dependency of Helios-positive population among CD4+ murine splenocytes and CD4+/CD8- murine thymocytes (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).
Flow Cytometry Analysis: A representative lot detected increased Helios-positive population among the TGF-beta-stimulated Treg, but not Teff cells from murine peripheral lymph node (LN) and human PBMCs (Thornton, A.M., et al. (2010). J Immunol.184(7):3433-3441).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-Helios Antibody, clone 22F6 is validated for use in Flow Cytometry for the detection of Helios.

Qualidade

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Helios in human PBMCs.

Descrição-alvo

~ 59 kDa calculated

forma física

Format: Purified

Protein G Purified

Purified Armenian hamster monoclonal IgG antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable